@article{scutteri_minimal_2025,
	title = {Minimal shuttle vectors for \textit{Saccharomyces cerevisiae}},
	volume = {10},
	rights = {https://creativecommons.org/licenses/by-nc-nd/4.0/},
	issn = {2397-7000},
	url = {https://academic.oup.com/synbio/article/doi/10.1093/synbio/ysaf010/8139874},
	doi = {10.1093/synbio/ysaf010},
	abstract = {Abstract 
            Sophisticated genetic engineering tasks such as protein domain grafting and multi-gene fusions are hampered by the lack of suitable vector backbones. In particular, many restriction sites are in the backbone outside the polylinker region (multiple cloning site; {MCS}) and thus unavailable for use, and the overall length of a plasmid correlates with poorer ligation efficiency. To address this need, we describe the design and validation of a collection of six minimal integrating or centromeric shuttle vectors for Saccharomyces cerevisiae, a widely used model organism in synthetic biology. We constructed the plasmids using de novo gene synthesis and consisting only of a yeast selection marker ({HIS}3, {LEU}2, {TRP}1, {URA}3, {KanMX}, or {natMX}6), a bacterial selection marker (ampicillin resistance), an origin of replication, and the {MCS} flanked by M13 forward and reverse sequences. We used truncated variants of these elements where available and eliminated all other sequences typically found in plasmids. The {MCS} consists of ten unique restriction sites. To our knowledge, at sizes ranging from {\textasciitilde}2.6 to 3.5 kb, these are the smallest shuttle vectors described for yeast. Further, we removed common restriction sites in the open reading frames and terminators, freeing up {\textasciitilde}30 cut sites in each plasmid. We named our {pLS} series in accordance with the well-known {pRS} vectors, which are on average 63\% larger: {pLS}400, {pLS}410 ({KanMX}); {pLS}403, {pLS}413 ({HIS}3); {pLS}404, {pLS}414 ({TRP}1); {pLS}405, {pLS}415 ({LEU}2); {pLS}406, {pLS}416 ({URA}3); and {pLS}408, {pLS}418 ({natMX}6). This resource substantially simplifies advanced synthetic biology engineering in S. cerevisiae.},
	pages = {ysaf010},
	number = {1},
	journaltitle = {Synthetic Biology},
	author = {Scutteri, Lorenzo and Barth, Patrick and Rahi, Sahand Jamal},
	urldate = {2025-08-14},
	date = {2025-02-14},
	langid = {english},
}